The term ‘Two-Spirit’ was invented in 1990.
The term ‘Two-Spirit’ – often abbreviated to ‘2S’, and particularly common in Canada – is a modern invention. It was first coined at the Indigenous Lesbian and Gay International Gathering in Winnipeg, Manitoba, in 1990 .
 de Vries, K. M. (2009). Berdache (Two-Spirit). In O’Brien, J. (ed.): Encyclopedia of gender and society. Los Angeles: SAGE. [Link]